CD14 antibody (AA 65-327)

Catalog No. ABIN4950462

This Rabbit Polyclonal antibody specifically detects CD14 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD14 antibody (AA 65-327) (ABIN4950462)

Target: CD14

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD14 Antibody

Binding Specificity: AA 65-327

Purification: Antigen affinity

Immunogen: Amino acids 65-327 of human CD14 were used as the immunogen for the CD14 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CD14 antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CD14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades