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GAPDH antibody (AA 62-91)

Name: GAPDH antibody (AA 62-91)
SKU: ABIN3029800
Price: 606.92 USD
Availability: InStock

GAPDH Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

Western blot analysis of lysate from A375 cell line using anti-GAPDH antibody diluted at 1:500.

IHC analysis of FFPE human hepatocarcinoma tissue stained with anti-GAPDH antibody

IHC

Anti-GAPDH antibody confocal immunofluorescent analysis with HeLa cell.

Confocal immunofluorescent analysis of anti-GAPDH antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

IHC

Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using anti-GAPDH antibody diluted at 1:1000 for each lane.

Western blot analysis of anti-GAPDH antibody and 1) A2058, 2) A375, and 3) CEM lysate

10 images

Catalog No. ABIN3029800

Datasheet as PDF

Target See all GAPDH Antibodies

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Binding Specificity
37

21

13

9

7

6

6

5

4

4

3

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 62-91
Reactivity
222

142

135

75

65

55

36

28

23

21

20

19

18

16

12

12

11

10

9

8

6

4

4

4

4

3

3

3

3

3

2

2

1

1

1

1

1
Human
Host
169

97

17

10

4
Rabbit
Compatible Secondaries
Clonality
190

105
Polyclonal
Conjugate
156

36

23

17

5

5

5

5

5

4

4

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
This GAPDH antibody is un-conjugated
Application
244

97

83

69

46

41

41

35

16

16

15

15

8

6

5

4

4

3

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purification

Purified

Immunogen

A portion of amino acids 62-91 from the human protein was used as the immunogen for this anti-GAPDH antibody.

Isotype

Ig Fraction

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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH Reactivity: Human WB, IF, IHC (p), ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Cow, Various Species WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH Reactivity: Human, Mouse, Rat WB, IF, IHC (p) Host: Mouse Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH Reactivity: Human, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 233-259), (C-Term) antibody
GAPDH Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB16543 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the anti-GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500-1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the anti-GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name

GAPDH (GAPDH Products)

Synonyms

G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

Background

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

UniProt

P04406