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Vimentin antibody (AA 430-457)

Name: Vimentin antibody (AA 430-457)
SKU: ABIN3029594
Price: 649.42 USD
Availability: InStock

The Rabbit Polyclonal anti-Vimentin antibody has been validated for WB, IHC, ELISA, IF and FACS. It is suitable to detect Vimentin in samples from Human.

Western blot analysis of anti-Vimentin antibody and NCI-H460 lysate (Vimentin antibody (AA 430-457))

Anti-Vimentin antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). (Vimentin antibody (AA 430-457))

FACS

Anti-Vimentin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue. (Vimentin antibody (AA 430-457))

IHC

Fluorescent confocal image of SY5Y cells stained with anti-Vimentin antibody at 1:100. (Vimentin antibody (AA 430-457))

IHC

FACS

7 images

Catalog No. ABIN3029594

Quick Overview for Vimentin antibody (AA 430-457) (ABIN3029594)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Discover our top product VIM Primary Antibody

Binding Specificity
34

32

17

16

12

10

9

7

5

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 430-457

Cross-Reactivity (Details)

Expected species reactivity: Bovine, Chicken, Hamster, Mouse, Pig, Primate, Rat, Xenopus

Purification

Purified

Immunogen

A portion of amino acids 430-457 from the human protein was used as the immunogen for this anti-Vimentin antibody.

Isotype

Ig Fraction
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the anti-Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:100,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the anti-Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

Vimentin (VIM)

Alternative Name

Vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis