CDC25A antibody (AA 271-300)

Catalog No. ABIN3030442

This Rabbit Polyclonal antibody specifically detects CDC25A in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CDC25A antibody (AA 271-300) (ABIN3030442)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CDC25A Antibody

Binding Specificity: AA 271-300

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 271-300 from the human protein was used as the immunogen for this Cdc25A antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Cdc25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at 4°C for up to one month. For long term, aliquot the Cdc25A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: Cdc25A

Background: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. [UniProt]

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase