ENOS antibody (AA 1156-1183)

Catalog No. ABIN3032003

The Rabbit Polyclonal anti-ENOS antibody has been validated for WB, ELISA, IF and FACS. It is suitable to detect ENOS in samples from Human.

Quick Overview for ENOS antibody (AA 1156-1183) (ABIN3032003)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-ENOS Antibody

Binding Specificity: AA 1156-1183

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Pig

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 1156-1183 from the human protein was used as the immunogen for this eNos antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the eNos antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at 4°C for up to one month. For long term, aliquot the eNos antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: eNos

Background: ENos/NOS3 produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. [UniProt]

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling