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Vimentin antibody (AA 152-181)

Name: Vimentin antibody (AA 152-181)
SKU: ABIN3029593
Price: 649.42 USD
Availability: InStock

This Rabbit Polyclonal antibody specifically detects Vimentin in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human.

Western blot analysis of VIM antibody and A2058, A375 lysate. (Vimentin antibody (AA 152-181))

IHC analysis of FFPE human breast carcinoma tissue stained with VIM antibody (Vimentin antibody (AA 152-181))

IHC

VIM antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). (Vimentin antibody (AA 152-181))

FACS

Western blot analysis of VIM antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2). (Vimentin antibody (AA 152-181))

VIM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue. (Vimentin antibody (AA 152-181))

IHC

FACS

8 images

Catalog No. ABIN3029593

Quick Overview for Vimentin antibody (AA 152-181) (ABIN3029593)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Discover our top product VIM Primary Antibody

Binding Specificity
34

32

17

16

12

10

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2

2

2

2

2

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2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

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1

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1

1

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1
AA 152-181

Cross-Reactivity (Details)

Expected species reactivity: Bovine, Hamster, Mouse, Pig, Rat

Purification

Purified

Immunogen

A portion of amino acids 152-181 from the human protein was used as the immunogen for this VIM antibody.

Isotype

Ig Fraction
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the VIM antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

For long term storage, aliquot the VIM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

Vimentin (VIM)

Alternative Name

VIM

Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis