Recombinant Caspase 3 antibody

Catalog No. ABIN4953301

The Rabbit Monoclonal anti-Caspase 3 antibody is suitable to detect Caspase 3 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant Caspase 3 antibody (ABIN4953301)

Target: Caspase 3 (CASP3)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RM250

Product Details anti-Caspase 3 Antibody

Characteristics: This recombinant Caspase-3 antibody reacts to human full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa).

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A peptide corresponding to residues within human Caspase-3 subunit p17 was used as the immunogen for this recombinant Caspase-3 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (FFPE): 1:1000-1:2500 (1),Western Blot: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant Caspase-3 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background: This recombinant Caspase-3 antibody reacts to human full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa).

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation