Recombinant MCAM antibody (C-Term)

Catalog No. ABIN4953302

This anti-MCAM antibody is a Rabbit Monoclonal antibody detecting MCAM in IHC and WB. Suitable for Human.

Quick Overview for Recombinant MCAM antibody (C-Term) (ABIN4953302)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: RM249

Product Details anti-MCAM Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Characteristics: This recombinant CD146 antibody reacts to human CD146 (Cell surface glycoprotein MUC18). It may also react to the mouse and rat protein, as predicted by immunogen homology.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A peptide corresponding to the residues near C-terminus of human CD146 was used as the immunogen for this recombinant CD146 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant CD146 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. IHC (FFPE): 1:200-1:500 (1),Western Blot: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant CD146 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background: This recombinant CD146 antibody reacts to human CD146 (Cell surface glycoprotein MUC18). It may also react to the mouse and rat protein, as predicted by immunogen homology.

Gene ID: 4162

UniProt: P43121