This recombinant H2BK23ac antibody reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.
Purification
Protein A purified from animal origin-free supernatant
Immunogen
An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys23) was used as the immunogen for this recombinant H2BK23ac antibody.
Reactivity: Human
WB, ChIP, DB, ChIP-seq
Host: Rabbit
Polyclonal
unconjugated
Application Notes
The stated application concentrations are suggested starting points. Titration of the recombinant H2BK23ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL
Restrictions
For Research Use only
Buffer
1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the recombinant H2BK23ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Target
Histone H2B
Background
This recombinant H2BK23ac antibody reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.