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Recombinant Histone 3 antibody (meLys79)

This Rabbit Monoclonal antibody specifically detects Histone 3 in WB, ELISA and ChIP. It exhibits reactivity toward All Species.
Catalog No. ABIN4953342

Quick Overview for Recombinant Histone 3 antibody (meLys79) (ABIN4953342)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Antibody Type

Recombinant Antibody

Reactivity

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All Species

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This Histone 3 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Clone

RM147
  • Binding Specificity

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    meLys79

    Characteristics

    This recombinant H3K79me1 antibody reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3) or other methylations of Histone H3.

    Purification

    Protein A purified from animal origin-free supernatant

    Immunogen

    A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79me1 antibody.

    Isotype

    IgG
  • Application Notes

    The stated application concentrations are suggested starting points. Titration of the recombinant H3K79me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.2-1 μg/mL,ChIP: 2-10 μg/mg of lysate,ELISA: 0.2-1 μg/mL

    Restrictions

    For Research Use only
  • Buffer

    1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store the recombinant H3K79me1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    Histone H3

    Background

    This recombinant H3K79me1 antibody reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3) or other methylations of Histone H3.

    Gene ID

    8350

    UniProt

    P84243
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