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Recombinant Histone 3 antibody (H3T3ph)

This Rabbit Monoclonal antibody specifically detects Histone 3 in WB, ELISA and ICC. It exhibits reactivity toward Various Species.

Western blot test of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3. (Recombinant Histone 3 antibody (H3T3ph))

ICC/IF of HeLa cells using recombinant phospho-Histone H3 antibody (red). (Recombinant Histone 3 antibody (H3T3ph))

ICC

2 images

Catalog No. ABIN4953411

$672.69

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Quick Overview for Recombinant Histone 3 antibody (H3T3ph) (ABIN4953411)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Antibody Type

Recombinant Antibody

Reactivity

1725
1152
1018
48
40
37
36
32
25
23
21
20
8
8
6
6
5
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Various Species

Host

Rabbit

Clonality

Monoclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

1380
593
511
416
347
309
207
206
205
188
177
121
114
49
48
37
26
25
19
18
18
7
7
7
4
3
2
1
1
1
1

Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone

RM159

Discover our top product H3 Primary Antibody

Binding Specificity
69

52

52

51

48

48

47

47

45

40

39

39

35

32

32

32

31

29

27

27

26

25

24

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22

20

19

18

18

17

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12

11

11

11

11

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10

10

10
H3T3ph

Purpose

Recombinant phospho-Histone H3 Antibody (Thr3)

Characteristics

This recombinant phospho-Histone H3 antibody reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones

Purification

Protein A purified from animal origin-free supernatant

Immunogen

A phospho-peptide corresponding to phospho-Histone H3 (Thr3) was used as the immunogen for this recombinant phospho-Histone H3 antibody.

Isotype

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store the recombinant phospho-Histone H3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

Histone H3

Background

This recombinant phospho-Histone H3 antibody reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones

UniProt

P84243