Recombinant Phosphotyrosine antibody

Catalog No. ABIN4953419

The Rabbit Monoclonal anti-Phosphotyrosine antibody (Clone RM111) (ABIN4953419) specifically detects Phosphotyrosine in WB, IP, IHC, ICC and ChIP.

Quick Overview for Recombinant Phosphotyrosine antibody (ABIN4953419)

Target: Phosphotyrosine

Antibody Type: Recombinant Antibody

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Clone: RM111

Product Details anti-Phosphotyrosine Antibody

Purpose: Recombinant Phosphotyrosine Antibody

Characteristics: This recombinant Phosphotyrosine antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine seen.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: Phosphotyrosine-BSA conjugate was used as the immunogen for this recombinant Phosphotyrosine antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Phosphotyrosine antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant Phosphotyrosine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: This recombinant Phosphotyrosine antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine seen.