Recombinant SMAD4 antibody (C-Term)

Catalog No. ABIN4953422

This anti-SMAD4 antibody is a Rabbit Monoclonal antibody detecting SMAD4 in WB and IHC. Suitable for Human.

Quick Overview for Recombinant SMAD4 antibody (C-Term) (ABIN4953422)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RM277

Product Details anti-SMAD4 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Characteristics: This recombinant SMAD4 antibody reacts to human SMAD4 and may also react to mouse or rat Smad4, as predicted by immunogen homology.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A peptide corresponding to the C-terminus of human SMAD4 was used as the immunogen for this recombinant SMAD4 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. IHC (FFPE): 1:1000-1:2000 (1),Western Blot: 1:500-1:1000

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant SMAD4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: This recombinant SMAD4 antibody reacts to human SMAD4 and may also react to mouse or rat Smad4, as predicted by immunogen homology.

Gene ID: 4089

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy