alpha Fetoprotein antibody (AA 300-329)

Catalog No. ABIN3029916

The Rabbit Polyclonal anti-alpha Fetoprotein antibody (ABIN3029916) specifically detects alpha Fetoprotein in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for alpha Fetoprotein antibody (AA 300-329) (ABIN3029916)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-alpha Fetoprotein Antibody

Binding Specificity: AA 300-329

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 300-329 from the human protein was used as the immunogen for this AFP antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the AFP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the AFP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Background: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process