ASK1 antibody (AA 944-973)

Catalog No. ABIN3031853

The Rabbit Polyclonal anti-ASK1 antibody has been validated for WB and ELISA. It is suitable to detect ASK1 in samples from Human.

Quick Overview for ASK1 antibody (AA 944-973) (ABIN3031853)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ASK1 Antibody

Binding Specificity: AA 944-973

Cross-Reactivity (Details): Expected species reactivity: Mouse

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 944-973 from the human protein was used as the immunogen for this ASK1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the ASK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the ASK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1

Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. ASK1/MAPKKK5/MAP3K5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that transcript is abundantly expressed in human heart and pancreas. The MAP3K5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAP3K5 does not activate MAPK/ERK.

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response