ATG12 antibody (AA 1-50)

Catalog No. ABIN3030084

This anti-ATG12 antibody is a Rabbit Polyclonal antibody detecting ATG12 in WB, ELISA and IF. Suitable for Human and Mouse.

Quick Overview for ATG12 antibody (AA 1-50) (ABIN3030084)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-ATG12 Antibody

Binding Specificity: AA 1-50

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 1-50 from the human protein was used as the immunogen for this ATG12 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the ATG12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the ATG12 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

APG12L/ATG12 is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.

UniProt: O94817

Pathways: Autophagy