Beclin 1 antibody (AA 210-239)

Catalog No. ABIN3030156

This anti-Beclin 1 antibody is a Rabbit Polyclonal antibody detecting Beclin 1 in WB, ELISA, IHC and FACS. Suitable for Human and Mouse.

Quick Overview for Beclin 1 antibody (AA 210-239) (ABIN3030156)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Beclin 1 Antibody

Binding Specificity: AA 210-239

Cross-Reactivity (Details): Expected species reactivity: Bovine, Pig, Rat

Purification: Purified

Immunogen: A portion of amino acids 210-239 from the human protein was used as the immunogen for this Beclin antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Beclin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Beclin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin 1

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.

UniProt: Q14457

Pathways: Autophagy