CD73 antibody (AA 520-550)

Catalog No. ABIN3029822

The Rabbit Polyclonal anti-CD73 antibody is suitable to detect CD73 in samples from Human and Mouse. It has been validated for WB, IHC and ELISA.

Quick Overview for CD73 antibody (AA 520-550) (ABIN3029822)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CD73 Antibody

Binding Specificity: AA 520-550

Purification: Purified

Immunogen: A portion of amino acids 520-550 from the human protein was used as the immunogen for this CD73 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CD73 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-2000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CD73 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background: Ecto-5-prime-nucleotidase/5-prime-ribonucleotide phosphohydrolase/NT5E/CD73 catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E/CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process