CDC25A antibody (AA 57-84)

Catalog No. ABIN3030443

The Rabbit Polyclonal anti-CDC25A antibody is suitable to detect CDC25A in samples from Human. It has been validated for WB and ELISA.

Quick Overview for CDC25A antibody (AA 57-84) (ABIN3030443)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CDC25A Antibody

Binding Specificity: AA 57-84

Cross-Reactivity (Details): Expected species reactivity: Bovine, Mouse, Rat

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 57-84 from the human protein was used as the immunogen for this Cdc25A antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Cdc25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Cdc25A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: Cdc25A

Background: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase