Cyclin D1 antibody (AA 203-238)

Catalog No. ABIN3029709

This anti-Cyclin D1 antibody is a Rabbit Polyclonal antibody detecting Cyclin D1 in WB, ELISA and IF. Suitable for Human.

Quick Overview for Cyclin D1 antibody (AA 203-238) (ABIN3029709)

Target: Cyclin D1 (CCND1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Cyclin D1 Antibody

Binding Specificity: AA 203-238

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 203-238 from the human protein was used as the immunogen for this Cyclin D antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Cyclin D antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Cyclin D antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Background: CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling