Cyclin D3 antibody (AA 33-66)

Catalog No. ABIN3030599

The Rabbit Polyclonal anti-Cyclin D3 antibody has been validated for WB and ELISA. It is suitable to detect Cyclin D3 in samples from Human, Mouse and Rat.

Quick Overview for Cyclin D3 antibody (AA 33-66) (ABIN3030599)

Target: Cyclin D3 (CCND3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cyclin D3 Antibody

Binding Specificity: AA 33-66

Purification: Antigen affinity purified

Immunogen: This Cyclin D3 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 33-66 amino acids from the N-terminal region of rat Ccnd3.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Cyclin D3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Cyclin D3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Cyclin D3

Target: Cyclin D3 (CCND3)

Background: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

UniProt: P48961

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process