Cyclin D3 antibody (AA 33-66)
Quick Overview for Cyclin D3 antibody (AA 33-66) (ABIN3030599)
Target
See all Cyclin D3 (CCND3) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 33-66
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Purification
- Antigen affinity purified
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Immunogen
- This Cyclin D3 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 33-66 amino acids from the N-terminal region of rat Ccnd3.
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Isotype
- Ig Fraction
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Application Notes
- Titration of the Cyclin D3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500-1:1000
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Aliquot the Cyclin D3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- Cyclin D3 (CCND3)
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Background
- Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
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UniProt
- P48961
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Pathways
- Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process
Target
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