DDX58 antibody (AA 585-614)

Catalog No. ABIN3030700

This Rabbit Polyclonal antibody specifically detects DDX58 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for DDX58 antibody (AA 585-614) (ABIN3030700)

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX58 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDX58 Antibody

Binding Specificity: AA 585-614

Purpose: DDX58 Antibody / RIG-I

Purification: Purified

Immunogen: A portion of amino acids 585-614 from the human protein was used as the immunogen for this DDX58 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the DDX58 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the DDX58 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for DDX58

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Alternative Name: DDX58

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, RIG-I/DDX58 triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). This protein is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

UniProt: O95786

Pathways: Activation of Innate immune Response, Hepatitis C