Dihydrofolate Reductase antibody (AA 1-50)

Catalog No. ABIN3028616

The Rabbit Polyclonal anti-Dihydrofolate Reductase antibody has been validated for WB, ELISA and IHC. It is suitable to detect Dihydrofolate Reductase in samples from Human, Mouse and Rat.

Quick Overview for Dihydrofolate Reductase antibody (AA 1-50) (ABIN3028616)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Dihydrofolate Reductase Antibody

Binding Specificity: AA 1-50

Cross-Reactivity (Details): Expected species reactivity: Bovine, Pig

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 1-50 from human Dihydrofolate reductase was used as the immunogen for this DHFR antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:25,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the DHFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR

Background: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases