ErbB2/Her2 antibody (AA 1236-1268)

Catalog No. ABIN3029492

The Rabbit Polyclonal anti-ErbB2/Her2 antibody has been validated for WB and ELISA. It is suitable to detect ErbB2/Her2 in samples from Mouse and Rat.

Quick Overview for ErbB2/Her2 antibody (AA 1236-1268) (ABIN3029492)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: AA 1236-1268

Purification: Antigen affinity purified

Immunogen: This mouse Erbb2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1236-1268 amino acids from the mouse Erbb2.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Erbb2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Erbb2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: Erbb2

Background: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity).

UniProt: P70424

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development