MAPK7 antibody (AA 776-805)

Catalog No. ABIN3031834

This Rabbit Polyclonal antibody specifically detects MAPK7 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for MAPK7 antibody (AA 776-805) (ABIN3031834)

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MAPK7 Antibody

Binding Specificity: AA 776-805

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine

Purification: Purified

Immunogen: A portion of amino acids 776-805 from the human protein was used as the immunogen for this ERK5 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the ERK5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the ERK5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MAPK7

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Alternative Name: ERK5

Background: MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.

UniProt: Q13164

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling