GAPDH antibody (AA 233-259)

Catalog No. ABIN3031092

The Rabbit Polyclonal anti-GAPDH antibody (ABIN3031092) specifically detects GAPDH in WB, IHC, ELISA, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for GAPDH antibody (AA 233-259) (ABIN3031092)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-GAPDH Antibody

Binding Specificity: AA 233-259

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Pig, Chicken

Purification: Purified

Immunogen: A portion of amino acids 233-259 from the human protein was used as the immunogen for this GAPDH antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

UniProt: P04406