GCLC antibody (AA 1-30)

Catalog No. ABIN3031076

The Rabbit Polyclonal anti-GCLC antibody is suitable to detect GCLC in samples from Human. It has been validated for WB, IHC, ELISA, IF and FACS.

Quick Overview for GCLC antibody (AA 1-30) (ABIN3031076)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-GCLC Antibody

Binding Specificity: AA 1-30

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this GCLC antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the GCLC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the GCLC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC

Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

UniProt: P48506

Pathways: Cell RedoxHomeostasis