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HIST1H4A antibody (H4K20me1)

This Rabbit Polyclonal antibody specifically detects HIST1H4A in ELISA, WB, IHC, FACS and DB. It exhibits reactivity toward Human.

IHC

Dot blot analysis of H4 antibody (Methyl 2/methylation-specific). (HIST1H4A antibody (H4K20me1))

H4 antibody flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control (top histogram). (HIST1H4A antibody (H4K20me1))

FACS

Western blot analysis of lysate from K562 cell line using H4 antibody diluted at 1:1000 for each lane. (HIST1H4A antibody (H4K20me1))

FACS

7 images

Catalog No. ABIN3031210

$612.54

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Quick Overview for HIST1H4A antibody (H4K20me1) (ABIN3031210)

Target

See all HIST1H4A Antibodies

HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This HIST1H4A antibody is un-conjugated

Application

ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Dot Blot (DB)

Binding Specificity
6

5

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
H4K20me1

Purpose

H4 Antibody / Methyl-Lys20 specific

Purification

Antigen affinity

Immunogen

Amino acids surrounding the K20 methylation site of the human protein were used as the immunogen for this Histone H4 antibody.

Isotype

Ig Fraction
anti-Histone Cluster 1, H4a (HIST1H4A) (2meLys20), (AA 1-30) antibody
HIST1H4A Reactivity: Human WB, FACS, DB, IHC (p) Host: Rabbit Polyclonal RB17115 unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K16ac) antibody
HIST1H4A Reactivity: Human ELISA, WB, IF, ChIP, ICC Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K31ac) antibody
HIST1H4A Reactivity: Human ELISA, WB, IF, ChIP, ICC Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K12ac) antibody
HIST1H4A Reactivity: Human ELISA, IF, ChIP, ICC Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K8ac) antibody
HIST1H4A Reactivity: Human ELISA, IF, ChIP, ICC Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K79ac) antibody
HIST1H4A Reactivity: Human ELISA, IF, ChIP, ICC Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (AA 1-30), (N-Term) antibody
HIST1H4A Reactivity: Human, Mouse WB Host: Rabbit Polyclonal RB17117 unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K5me1) antibody
HIST1H4A Reactivity: Human ELISA, IF, ChIP Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (meArg3) antibody
HIST1H4A Reactivity: Human ELISA, WB, IF Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (AA 1-12) antibody
HIST1H4A Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

Titration of the H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Aliquot the H4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Target

HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Alternative Name

H4

Background

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.

UniProt

P62805