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HIF1A antibody (AA 519-547)

Name: HIF1A antibody (AA 519-547)
SKU: ABIN3031284
Price: 649.42 USD
Availability: InStock

HIF1A Reactivity: Human, Mouse WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

Western blot analysis of HIF1A antibody and mouse NIH3T3 lysate (HIF1A antibody (AA 519-547))

Confocal immunofluorescent analysis of HIF1A antibody with MDA-MB231 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (HIF1A antibody (AA 519-547))

HIF1A antibody flow cytometric analysis of NIH3T3 cells (right histogram) compared to a negative control cell (left histogram). (HIF1A antibody (AA 519-547))

FACS

HIF1A antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma. (HIF1A antibody (AA 519-547))

IHC

FACS

7 images

Catalog No. ABIN3031284

Datasheet as PDF

Target See all HIF1A Antibodies

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
Binding Specificity
20

16

16

14

8

7

6

6

6

5

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 519-547
Reactivity
157

92

85

23

23

20

13

11

9

8

7

6

6

5

4

2

2

2

2

2

1

1

1

1

1

1
Human, Mouse
Host
151

49

5
Rabbit
Compatible Secondaries
Clonality
157

48
Polyclonal
Conjugate
103

24

16

6

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This HIF1A antibody is un-conjugated
Application
165

75

60

52

46

39

39

26

26

25

12

8

7

7

3

3

3

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Cross-Reactivity (Details)

Expected species reactivity: Bovine, Rat

Purification

Purified

Immunogen

A portion of amino acids 519-547 from the human protein was used as the immunogen for this HIF1A antibody.

Isotype

Ig Fraction

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anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 519-547) antibody
HIF1A Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB14548 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody
HIF1A Reactivity: Human WB, IHC, ELISA, ICC Host: Mouse Monoclonal 1A3 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 216-336) antibody
HIF1A Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 728-757) antibody
HIF1A Reactivity: Human, Mouse WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 703-732) antibody
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anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 329-530) antibody
HIF1A Reactivity: Human, Mouse, Rat, Cow, Dog WB, IHC, IP, IF, ICC Host: Mouse Monoclonal ESEE122 unconjugated

Application Notes

Titration of the HIF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the HIF1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name

HIF1A (HIF1A Products)

Background

HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. [UniProt]

UniProt

Q16665

Pathways

Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect