HNF4A antibody (AA 281-312)

Catalog No. ABIN3031189

This Rabbit Polyclonal antibody specifically detects HNF4A in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for HNF4A antibody (AA 281-312) (ABIN3031189)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-HNF4A Antibody

Binding Specificity: AA 281-312

Cross-Reactivity (Details): Expected species reactivity: Mouse

Purification: Purified

Immunogen: A portion of amino acids 281-312 from the human protein was used as the immunogen for this HNF4A antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the HNF4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the HNF4A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A

Background: Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProt]

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process