HSP27 antibody (AA 56-85)

Catalog No. ABIN3031143

This anti-HSP27 antibody is a Rabbit Polyclonal antibody detecting HSP27 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for HSP27 antibody (AA 56-85) (ABIN3031143)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HSP27 Antibody

Binding Specificity: AA 56-85

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 56-85 from the human protein was used as the immunogen for this HSPB1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the HSPB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the HSPB1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1

Background: In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling