IRF3 antibody (AA 46-72)

Catalog No. ABIN3031417

This Rabbit Polyclonal antibody specifically detects IRF3 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for IRF3 antibody (AA 46-72) (ABIN3031417)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IRF3 Antibody

Binding Specificity: AA 46-72

Cross-Reactivity (Details): Expected species reactivity: Bovine, Pig

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 46-72 from the mouse protein was used as the immunogen for this Irf3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Irf3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Irf3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: Irf3

Background: Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains (By similarity).

UniProt: P70671

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades