Kdm6b antibody (AA 1-30)

Catalog No. ABIN3031660

This Rabbit Polyclonal antibody specifically detects Kdm6b in WB, ELISA, IF and IHC. It exhibits reactivity toward Human.

Quick Overview for Kdm6b antibody (AA 1-30) (ABIN3031660)

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kdm6b antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-Kdm6b Antibody

Binding Specificity: AA 1-30

Cross-Reactivity (Details): Expected species reactivity: Mouse

Purification: Purified

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this JMJD3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the JMJD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the JMJD3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Kdm6b

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Alternative Name: JMJD3 (KDM6B)

Background: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

UniProt: O15054

Pathways: Warburg Effect