K-RAS antibody (AA 146-174)

Catalog No. ABIN3031120

This Rabbit Polyclonal antibody specifically detects K-RAS in WB, ELISA, IF and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for K-RAS antibody (AA 146-174) (ABIN3031120)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This K-RAS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-K-RAS Antibody

Binding Specificity: AA 146-174

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 146-174 from the human protein was used as the immunogen for this KRAS antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the KRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500-2000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the KRAS antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS

Background: This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

UniProt: P01116