K-RAS antibody (N-Term)

Catalog No. ABIN2786191

The Rabbit Polyclonal anti-K-RAS antibody is suitable to detect K-RAS in samples from Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for K-RAS antibody (N-Term) (ABIN2786191)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This K-RAS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-K-RAS Antibody

Binding Specificity: N-Term

Purpose: KRAS Antibody - N-terminal region

Sequence: TEYKLVVVGA GGVGKSALTI QLIQNHFVDE YDPTIEDSYR KQVVIDGETC

Specificity: 100 % homologous to human KRAS, NRAS and HRAS.

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against KRAS. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KRAS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55408-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS

Background:

Background Information: KRAS is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Gene Name: V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Alternative Symbols: NS, NS3, OES, CFC2, RALD, K-Ras, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras 2, C-K-RAS, c-Ki-ras, c-Ki-ras2

Protein Name: GTPase NRas

Molecular Weight: 22 kDa

Gene ID: 3845

NCBI Accession: NP_203524

UniProt: P01111