LC3B antibody (AA 89-122)

Catalog No. ABIN3031786

This Rabbit Polyclonal antibody specifically detects LC3B in ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for LC3B antibody (AA 89-122) (ABIN3031786)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-LC3B Antibody

Binding Specificity: AA 89-122

Cross-Reactivity (Details): Expected species reactivity: Bovine

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 89-122 from the human protein was used as the immunogen for this LC3B antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:10-1:100,Immunocytochemistry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the LC3B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: LC3B

Background: MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3B is one of the light chain subunits and can associate with either MAP1A or MAP1B. It is a Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). LC3B plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.

UniProt: Q9GZQ8

Pathways: Autophagy