LIN28A antibody (N-Term)

Catalog No. ABIN3031622

The Rabbit Polyclonal anti-LIN28A antibody has been validated for WB and ELISA. It is suitable to detect LIN28A in samples from Human.

Quick Overview for LIN28A antibody (N-Term) (ABIN3031622)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-LIN28A Antibody

Binding Specificity: N-Term

Purification: Antigen affinity purified

Immunogen: This LIN28A antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 22-56 amino acids from the N-terminal region of human LIN28A.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the LIN28A antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the LIN28A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Alternative Name: LIN28A

Background: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 [removed]By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

UniProt: Q9H9Z2

Pathways: Stem Cell Maintenance