LSD1 antibody (AA 457-490)

Catalog No. ABIN3031652

The Rabbit Polyclonal anti-LSD1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect LSD1 in samples from Human.

Quick Overview for LSD1 antibody (AA 457-490) (ABIN3031652)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LSD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-LSD1 Antibody

Binding Specificity: AA 457-490

Cross-Reactivity (Details): Expected species reactivity: Mouse

Purification: Purified

Immunogen: A portion of amino acids 457-490 from the human protein was used as the immunogen for this LSD1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the LSD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the LSD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Alternative Name: LSD1

Background: LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

UniProt: O60341

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect