MCM2 antibody (AA 649-677)

Catalog No. ABIN3031801

This Rabbit Polyclonal antibody specifically detects MCM2 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for MCM2 antibody (AA 649-677) (ABIN3031801)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MCM2 Antibody

Binding Specificity: AA 649-677

Cross-Reactivity (Details): Expected species reactivity: Xenopus

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 649-677 from the human protein was used as the immunogen for this MCM2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the MCM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-2000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MCM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2

Background: MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA