MECP2 antibody (AA 58-87)

Catalog No. ABIN3031763

The Rabbit Polyclonal anti-MECP2 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect MECP2 in samples from Human.

Quick Overview for MECP2 antibody (AA 58-87) (ABIN3031763)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-MECP2 Antibody

Binding Specificity: AA 58-87

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Primate

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 58-87 from the human protein was used as the immunogen for this MeCP2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the MeCP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MeCP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MeCP2

Background: MeCP2 is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). [UniProt]

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane