MAP2 antibody (pSer1539)

Catalog No. ABIN3031685

The Rabbit Polyclonal anti-MAP2 antibody has been validated for ELISA and DB. It is suitable to detect MAP2 in samples from Human.

Quick Overview for MAP2 antibody (pSer1539) (ABIN3031685)

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Product Details anti-MAP2 Antibody

Binding Specificity: pSer1539

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Purification: Antigen affinity purified

Immunogen: This phospho-MAP2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS1539 of human MAP2.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-MAP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-MAP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP2

Background: MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2, two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.

UniProt: P11137