PTEN antibody (pThr382)

Catalog No. ABIN3032205

Product Details anti-PTEN Antibody

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Binding Specificity: pThr382

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTEN antibody is un-conjugated

Application: Dot Blot (DB), ELISA

Cross-Reactivity (Details): Expected species reactivity: Mouse, Xenopus

Purification: Antigen affinity purified

Immunogen: This phospho-PTEN antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT382 of human PTEN.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-PTEN antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-PTEN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PTEN

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Alternative Name: PTEN (PTEN Products)

Background: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

UniProt: P60484

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Inositol Metabolic Process, Synaptic Membrane, Regulation of Cell Size, Autophagy, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling