PTEN antibody (N-Term)

Catalog No. ABIN6242544

The Mouse Monoclonal anti-PTEN antibody is suitable to detect PTEN in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for PTEN antibody (N-Term) (ABIN6242544)

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTEN antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1096CT4-3-3

Product Details anti-PTEN Antibody

Binding Specificity: AA 10-44, N-Term

Predicted Reactivity: X

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This PTEN antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 10-44 amino acids from the N-terminal region of human PTEN.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for PTEN

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Alternative Name: PTEN

Background: Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.

Molecular Weight: 47166

UniProt: P60484

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Inositol Metabolic Process, Synaptic Membrane, Regulation of Cell Size, Autophagy, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling