SMAD2 antibody (pSer118)

Catalog No. ABIN3032618

The Rabbit Polyclonal anti-SMAD2 antibody has been validated for ELISA, IF and DB. It is suitable to detect SMAD2 in samples from Human.

Quick Overview for SMAD2 antibody (pSer118) (ABIN3032618)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Dot Blot (DB)

Product Details anti-SMAD2 Antibody

Binding Specificity: pSer118

Cross-Reactivity (Details): Expected species reactivity: Bovine, Chicken, Drosophila, Mouse, Pig, Rat, Zebrafish

Purification: Antigen affinity purified

Immunogen: This phospho-SMAD2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS118 of human SMAD2.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:10-1:50,Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: Smad2

Background: The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus