YAP1 antibody (pSer127)

Catalog No. ABIN3029660

The Rabbit Polyclonal anti-YAP1 antibody has been validated for ELISA and DB. It is suitable to detect YAP1 in samples from Human.

Quick Overview for YAP1 antibody (pSer127) (ABIN3029660)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Product Details anti-YAP1 Antibody

Binding Specificity: pSer127

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Chicken

Purification: Antigen affinity purified

Immunogen: This phospho-YAP antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS127 of human YAP1.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-YAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-YAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP

Background: Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha