PML antibody (Sumoylation Site)

Catalog No. ABIN3032330

The Rabbit Polyclonal anti-PML antibody has been validated for WB, IHC, IF, ELISA and FACS. It is suitable to detect PML in samples from Human.

Quick Overview for PML antibody (Sumoylation Site) (ABIN3032330)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Product Details anti-PML Antibody

Binding Specificity: AA 470-499, Sumoylation Site

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 470-499 (sumoylation site) from the human protein was used as the immunogen for this PML antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PML antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PML antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML

Background: PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL).

UniProt: P29590

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus