RAC1 antibody

Catalog No. ABIN3032428

This Mouse Monoclonal antibody specifically detects RAC1 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for RAC1 antibody (ABIN3032428)

Target: RAC1

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 1301CT276-121-104

Product Details anti-RAC1 Antibody

Cross-Reactivity (Details): Expected species reactivity: Bovine, Mouse

Purification: Purified

Immunogen: This RAC1 antibody was produced from a mouse immunized with a KLH conjugated synthetic peptide from the human protein.

Isotype: IgG2b

Application Details

Application Notes: Titration of the RAC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Flow Cytometry: 1:100,IHC (Paraffin): 1:25,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the RAC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for RAC1

Target: RAC1

Background: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2, this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.

UniProt: P63000

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling