RUNX1 antibody (C-Term)

Catalog No. ABIN3032498

The Rabbit Polyclonal anti-RUNX1 antibody has been validated for WB and ELISA. It is suitable to detect RUNX1 in samples from Human and Mouse.

Quick Overview for RUNX1 antibody (C-Term) (ABIN3032498)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RUNX1 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity purified

Immunogen: This Runx1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 426-459 amino acids from the C-terminal region of mouse Runx1.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Runx1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Runx1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: Runx1

Background: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. Essential for the development of normal hematopoiesis. Isoform 4 shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B- dependent transcriptional activation (By similarity).

UniProt: Q03347