SETD8 antibody (N-Term)

Catalog No. ABIN3032551

This Mouse Monoclonal antibody specifically detects SETD8 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for SETD8 antibody (N-Term) (ABIN3032551)

Target: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SETD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 43AT551-86

Product Details anti-SETD8 Antibody

Binding Specificity: N-Term

Purification: Purified

Immunogen: This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07.

Isotype: IgG1

Application Details

Application Notes: Titration of the SET07 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SET07 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SETD8

Target: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))

Alternative Name: SET07

Background: SET07 is a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. [UniProt]

UniProt: Q9NQR1