Sonic Hedgehog antibody (AA 395-450)

Catalog No. ABIN3029025

This anti-Sonic Hedgehog antibody is a Rabbit Polyclonal antibody detecting Sonic Hedgehog in WB, ELISA and FACS. Suitable for Human and Mouse.

Quick Overview for Sonic Hedgehog antibody (AA 395-450) (ABIN3029025)

Target: Sonic Hedgehog (SHH)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: AA 395-450

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 395-450 were used as immunogen for this Shh antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Shh antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The 45 kDa precursor protein autocleaves into 27 kDa amino and 19 kDa carboxy fragments.\. Flow Cytometry: 1:25,Western blot: 1:1000 (1)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Shh antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Shh

Background: Sonic hedgehog binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO.

UniProt: Q62226

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development